Microfluidic solutions for the controlled production of monodisperse polymer nanoparticles such as PLGA, PEG, PVC.

Dolomite’s modular nanoparticle synthesis systems apply high shear micromixing and hydrodynamic focusing microfluidic methods for polymer particle production ranging in sizes from 50nm to 500nm.

The microfluidic technology of continuous and controllable laminar flow allows the production of high-yield and high-quality polymer nanoparticles. Plus, the superior control over the size, shape and morphology of particles enables greater reproducibility and scalability (up to kilos per month). These substantial improvements in nanoparticle generation are highly valued in pharmaceutical industry whilst compared to conventional batch methods.

 

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Microfluidic technology & benefits

 

Microfluidics, also known as a ‘lab-on-a-chip’, enables the precise control, manipulation and analysis of fluids in the microlitre to picolitre range. Furthermore, it allows the manipulation of living matter by mixing, separating and handling different components at a microscale.

Dolomite is pioneering the use of microfluidic devices for small-scale fluid control and analysis, enabling engineers and scientists to take full advantage of the following benefits:

Traditional methods (batch) Microfluidic method
Nanoparticle generation ~30%  ~100% 
Reagent distribution ~20% ~1%
Waste ~50% Near 0%
Reproducibility Low High
Surfactant mixing Uneven Homogenous
Particle size control Poor Precise

Nanoparticle synthesis

Robust, high-throughput methods of particle production in the size range of 50 to 500 nanometres have received significant interest within the scientific and pharmaceutical communities due to a wide variety of emerging applications in the fields of targeted drug delivery and controlled drug release. Scale-up of the particle fabrication process using batch techniques typically results in a reduction of control over the synthesis process, leading to wide particles size distributions and, in some cases, to uncontrolled particle aggregation.

By contrast, the use of microfluidic devices for nanoparticle synthesis brings advantages such as: enhanced control over each stage of particle fabrication process, greater particle yields, and ease of scale-up. This continuous flow methodology can be applied to produce high grade PLGA nanoparticles with satisfying criteria such as: quantity of residual solvent present, presence of processing aids and high degree of batch-to-batch consistency.

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Microfluidic Nanoparticle Generation System

System specifications

Particle size

Particles ranging from 50 nm to 500 nm 

Particle monodispersity

Extremely monodisperse nanoparticles (CV down to 20-30%

Particle generation frequency Up to 10 MHz for a standard system and 1 GHz for a production system (depending on reagents and particle sizes) 
Flow rate range

From 5 nL/min to > 100 mL/min (depending on solution properties) 

Flow pressure

Flexible with vacuum and pressure capability in the same pump (pump pressure range: 0-10 bar). Pumps contain integrated reservoir to eliminate the risk of vial fracture in operation

Chemical resistance

Very high (wetted materials: glass, PEEK, ETFE, FEP, PTFE)

 

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Microfluidic High-Throughput System

Effortless production scale-up

Telos® High-Throughput Production Systems provide a controlled solution for scaling-up droplet, particles or emulsion manufacturing. Each Dolomite Microfluidic System has a flexible configuration which allows up to 10 Telos® Clamp Modules to be assembled in parallel, enabling identical conditions for scale-up of production rate for up to 500,000 monodisperse particles per second.

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Further reading

Methods of diverse API encapsulation in polymer beads
Microfluidics blog

Methods of diverse API encapsulation in polymer beads

Read about microfluidic methods to encapsulate organic soluble and water soluble APIs in PLGA particles.

 Continuous microfluidic synthesis of PLGA nanoparticles by micromixing
Application Notes

Continuous microfluidic synthesis of PLGA nanoparticles by micromixing

Methodology for production of monodisperse PLGA nanoparticles in sizes ranging from 42 nm to 95 nm using micromixing chip.

Design and self-assembly of PBLG-b-ELP hybrid diblock copolymers based on synthetic and elastin-like polypeptides
Publications

Design and self-assembly of PBLG-b-ELP hybrid diblock copolymers based on synthetic and elastin-like polypeptides

Read about how the precision synthesis and self-assembly of amphiphilic copolypeptides containing a recombinant elastin-like polypeptide (ELP) block used as a macroinitiator for the ring opening polymerization (ROP) of γ-benzyl-L-glutamate (γ-BLG NCA).

Interested to learn more about this system?

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