Providing a modular platform for protocol development and formulation library generation. 

Dolomite presents a next-generation system dedicated to protocol development and production of a wide range of nanoparticle types including liposomes, lipid nanoparticles and more. Tailored and scalable, it facilitates library generation for formulation screening allowing you to progress through the protocol development pipeline without the need to change your entire system. 

 

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Liposome and Lipid Nanoparticle Synthesis

The ability to produce monodisperse liposomes and lipid nanoparticles with narrow size distribution and acutely control the reproducibility is crucial for the scientific and pharmaceutical communities. Within the fields of targeted drug delivery and controlled drug release, parameters like particle size can affect the rate of drug uptake and clearance, which emphasises the need for a robust, scalable fabrication process. Batch techniques typically result in a reduction of control over the synthesis process, leading to wide particles size distributions and, in some cases, to uncontrolled particle aggregation. 

By contrast, microfluidic encapsulation methods have demonstrated the potential for achieving higher control over the physical properties of the final nano-lipid or liposome product than conventional batch methods. Typical microfluidics characteristics, such as low Reynolds number and diffusion dominated mass transfer, make it the most viable method for producing lipid-based nanoscale vesicular systems with the potential for clinical application.

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Introduction to the Automated Nanoparticle System

System specifications

Particle size

Particles ranging from 40 nm to 800 nm.

Particle monodispersity

Extremely monodisperse with PDI ≈ 0.1 – 0.2.

Sample volume Min sample volume 200 ul; the valve allows accurate collection of the sample with the option to extract the heart cut – the central cut of the sample. Continuous mode of operation allows the production of larger sample volumes.
Automation

The system allows the creation of protocols, which can: 

  • calculate the volume required for the entered FRR
  • correct the timing ensuring correct reagent convergence in the chip
  • collect correct sample volume using the Automated Sample Valve
  • wash and prime the system
  • run multiple experiments with one load
Flexibility

The modular system can be paired with a range of glass chips, syringes and sample loops to be tailored to your specific application.

Chemical resistance

Very high (wetted materials: glass, PEEK, ETFE, FEP, PTFE).

 

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Further reading

Flyer

Ready to take your nanoparticle generation process to the next level? Read our flyer to learn how Dolomite can help you minimize the cost and optimize your protocols quicker

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Application note – Controlled synthesis of lipid nanoparticles using the ANP System

 

Research of key parameters involved in tuning LNPs’ size, and a comparison between the protocol and continuous mode to demonstrate the transferability of the process parameters for bulk production of LNPs.

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Optimising LNPs for novel nanomedicines

 

Discover our scientific poster where we investigate flow rate ratio (FRR) with the ANP System to generate fine-tuned LNPs.

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Maintaining LNP performance when increasing throughput

 

In this scientific poster we demonstrate the effect of total flow rate (TFR) on the LNP size and polydispersity index (PDI).

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Develop and produce LNPs with a single system solution

 

Discover our scientific poster where we investigate the transferability of process parameters from protocol to continuous generation of LNPs.

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Secret ingredients behind the breakthrough Covid vaccines
PUBLICATION

Secret ingredients behind the breakthrough Covid vaccines

Lipid Delivery Systems for Nucleic-Acid-Based-Drugs: From Production to Clinical Applications
PUBLICATION

Lipid Delivery Systems for Nucleic-Acid-Based-Drugs: From Production to Clinical Applications

mRNA vaccine delivery using lipid nanoparticles
PUBLICATION

mRNA vaccine delivery using lipid nanoparticles

Interested to learn more about this system?