µEncapsulator 2 Reagent Droplet Chip

The µEncapsulator 2 Reagent Droplet Chip (hydrophilic) enables in vitro evolution, by forming millions of picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution. Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 2 Reagent fluorophilic chip, to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator hydrophilic chip. The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.

The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.

The µEncapsulator 2 Reagent Droplet Chip (hydrophilic) enables in vitro evolution, by forming millions of picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution. Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 2 Reagent fluorophilic chip, to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator hydrophilic chip. The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.

The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.

The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.

The µEncapsulator 2 Reagent Droplet Chip (hydrophilic) enables in vitro evolution, by forming millions of picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution. Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 2 Reagent fluorophilic chip, to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator hydrophilic chip. The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.

The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.

The µEncapsulator 2 Reagent Droplet Chip (hydrophilic) enables in vitro evolution, by forming millions of picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution. Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 2 Reagent fluorophilic chip, to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator hydrophilic chip. The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.

The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.

The µEncapsulator 2 Reagent Droplet Chip (hydrophilic) enables in vitro evolution, by forming millions of picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution. Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 2 Reagent fluorophilic chip, to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator hydrophilic chip. The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.

The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.

Part number 3200528Part number 3200527Part number 3200530Part number 3200529Part number 3200445Part number 3200564Part number 3200563Part number 3200566Part number 3200565Part number 3200568Part number 3200567
Pack Size
Channel Size
Coating
Quantity
Unit Price
£510.00
£510.00
£510.00
£510.00
£510.00
£1,250.00
£1,250.00
£1,250.00
£1,250.00
£1,250.00
£1,250.00
  • Overview
  • VARIATIONS

Features & Benefits:

Generic features and benefits


  • Chip size 11.25mm x 15mm x 2mm
  • Attached FKM 0.5mm thick gasket for chip-to-chip connection
  • Integrated flow resistors for excellent flow stability
  • 100µm scale marks at the junction for imaging
  • Fabricated in glass
  • Very smooth channels (surface roughness Ra of 5 nm)
  • Excellent optical clarity
Hydrophilic features and benefits
  • Rapid generation of 2pl double emulsion droplets (an aqueous core, surrounded by an oil shell)
  • Channel cross–section at the junction 15µm x 20µm (depth x width)
  • Channel cross–section after the junction 15µm x 115µm (depth x width)
  • On–chip filter on droplet lines with pore size 15µm x 20µm (depth x width)
  • On–chip filter on carrier oil line with pore size 7.5µm x 20µm (depth x width)
Fluorophilic features and benefits
  • Rapid generation of 2 reagent droplets containing biological material
  • Channel cross-section at the junction 50 µm x 55 µm (depth x width)
  • Channel cross-section after the junction 50 µm x 150 µm (depth x width)
  • Fluorophilic surface coating for use with fluorocarbon carrier oils
  • F20* flow resistor on each droplet line
  • F600* flow resistor on oil line
  • On-chip filter on droplet lines with pore size 50µm x 55µm (depth x width)
  • On-chip filter on carrier oil line with pore size 25µm x 55µm (depth x width)

*F(X) notation signifies the approximate flow rate in µl/min that is obtained when water is pumped at 1 bar pressure at room temperature and there is no other fluidic resistance in series.

 

PART NUMBER MATERIAL CHANNEL SIZE COATING

3200527

3200565 (pack of 3)

Glass 15 µm Fluorophilic

3200528

3200566 (pack of 3)

Glass 15 µm Hydrophilic

3200529

3200567 (pack of 3)

Glass 30 µm Fluorophilic

3200530

3200568 (pack of 3)

Glass 30 µm Hydrophilic

3200445

3200563 (pack of 3)

Glass 50 µm Fluorophilic
3200564 (pack of 3) Glass 50 µm Hydrophilic

 

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