Knowledge Base

Recorded Webinar: Learn how to produce PLGA nanoparticles

Description

This webinar will introduce you novel Dolomite’s methods of PLGA nanoparticle production. It’s a practical session showing how you can create nanoparticles using microfluidics approach and explain benefits over traditional batch methods. By the end of the session, you’ll not only see the benefits but also realise how easily validate and expand your research with Dolomite Microfluidics.

Questions and Answers

1. Is it possible to work at 80°C?

Yes, there is a device called TCU-100 that enables work up to 100°C.

2. Is it possible to produce nanoparticles with a two-pump system?

Yes, but we would recommend 3 P-Pumps for both methodologies.

3. Does the solution need to be filtered before pumping into the chip?

Dependant of the size of particulates, for smaller than 5 µm – no problems!

4. What pressure is needed for 20 ul per min? 50 ul per min?

Depending on the flow resistance – from 200 mbar to 3000 mbar.

5. How long the hydrophobic cover remains stable? Do you need to cover it before each experiment?

The hydrophobic coating is permanent – no replacement is needed.

6. Can I use the 190 µm chip for nanoparticle production or is the 100 µm chip more efficient in controlling the size?

100 µm will be more effective, but in principle you can use the 190 µm chip.

7. When you quoted the flow rate, did you say if that was in flow of PLGA or outflow of the combined fluid?

PLGA flow – the total flow rate will be up to 5x higher.

8. Is there any clogging problem when liquid flows into the channels?

No – our protocols are specifically designed to avoid this issue.

9. Do you study for the fluid dynamics of your systems at different flow rates? Is mixing always laminar or do you also have secondary mixing effects?

During our testing, mixing was always laminar.

10.Would the hydrophilic coating of the chip still be retained with autoclave cycle (given the chip is autoclavable)?

Yes – the coating will not be affected by autoclave.

11. I’m facing continuous blockages when I’m using PLGA, do you recommend soaking the chip in HCL overnight to clean it?

No – we’d recommend flushing with a solvent e.g. acetone.

12.The flow rate of PLGA is 20 ul per min is in the 5-input chip. What are the flow rates of the DDE and acetone? In the 3-input chip, the flow rate of PLGA is 50 ul per min. What is the flow rate of the oil? What are the pressure of each inlet in this case?

This depends on the size required – the flow rates of each of the liquids will be different for each PLGA particle size.

13. Will the coating of the chip resist acid or basic treatments?

Low pH environments will etch glass along with the coating – so low pH is not recommended.

14. Can the chip coating be regenerated?

The cleaning protocol is solvent addition overnight – we would not recommend regenerating coating at user site – in principle however, it is possible.

15. How many pumps are required to run 10 chip Telos System? To get max throughout?

Same 3 P-Pump arrangement.

16. What is the cleaning protocol for reusing microfluidic chips?

Acetone flush overnight. The same cleaning protocol applies to 5-input chips and 3 input chips.

17. Is this system able to produce solid lipid nanoparticles?

Yes, in particular the 5-input microfluidic chip.

18. What is the maximum output when using the 3 pump system?

50 ul/min per channel of Acetone-PLGA solution.

19. Any useful tricks to remove dust from the microfluidic chip?

Best trick is to avoid getting dust into the chip by controlling the environment – if the chip is blocked flow backwards to dislodge dust particles.

20. When using the 3 pump system, what should the flow rate of the flushing fluid be?

Approximately 10 ul/min – its usage is minimal.

21. What is the maximum output when using the 3 pump system?

Approximately 10 ul/min – its usage is minimal.

22. When using Fluoro-phase, how do you remove it from the sample afterwards?

It is very heavy – so will separate under gravity.

23. What are the advantages of using a pressurized pump over the any other syringe or peristaltic pumps?

The precision flow and the lack of pulsation.

24. What solvents are Dolomite microfluidic chips compatible with?

Our chips and systems are chemically inert – the only restriction is high pH systems.

25. What is the typical volume of nanoparticles one can get after running the pumps for 5 hours @ 50ul/min?

15000 ul, the PLGA fraction will be 0.5-2.0 weight percent.

26. Are mentioned chemicals used for making the nanoparticles FDA approved? Can be used in the Pharma industry for large scale nanoparticles?

Yes – the components of Aqua-Phase are FDA approved for Pharma use.

27. What pressure is needed for making 80 nm particles (all ports) in the 3port/ 5 port chips?

That can be achieved using 200 mbar pressure or for better control over the conditions 2000 mbar- the pressure is altered by altering flow resistance leading to the microfluidic chip.