Screening of individual cells for an enzymatic activity or antibodies using fluorescence-activated cell sorting (FACS) is an efficient approach for library screening. An example of an application that can use FACS sorting of libraries is directed evolution for protein engineering, which can be very useful in activities such as improving antibodies and other therapeutic proteins.
Microfluidics allows screening on a single cell level, at high throughput, and most importantly for genotype-phenotype coupling. The small (picolitre) droplet volume means this method reduces assay volume, which ultimately leads to cost savings. The droplets compartmentalise individual cells entrapping all secreted molecules and analytes, preventing interference with other cells and products.
Since FACS devices work with aqueous buffers as sheath fluid, the upcoming webinar on Thursday, 17th of September will demonstrate the microfluidic methods for the production of double emulsions (water-in-oil-in-water) using Dolomite’s µEncapsulator system.
Read our Application Note to learn more about the production of a double emulsion on the µEncapsulator System using a 30µm µEncapsulator 1- 2 Reagent Droplet Chip. The document provides a comprehensive overview of the methodology and showcases the capabilities of the system.
This webinar introduced our Dolomite’s µEncapsulator System. It is an exceptional solution for this application as it enables the simple, quick and reliable encapsulation of single cells, DNA and/or functionalized beads in high precision, monodisperse picoliter droplets.