Microfluidics allows production of hydrogel particles in the range 5 µm to 500 µm diameter with exceptional control over size, shape and morphology. Generation of hydrogel beads using microfluidics also allows creation of more complex particles such as encapsulation/compartmentalisation of biological cells, DNA or nanoparticles inside each hydrogel bead or double emulsions for generating shelled particles and multicomponent droplet generation for Janus particles.
Dolomite droplet systems offer a range of benefits for hydrogel production:
- Excellent control: precisely control and optimisation of the size, shape and morphology
- Particle size range: create particles from 5 µm to 500 µm diameter ( +/- 10 %)
- Produce wide range of particle types: simple hydrogel beads, encapsulations, double emulsions or nanoparticles through water evaporation
- Systems for basic to advanced users: from basic starter systems ideal for initial trials, to advanced systems allowing complex upstream and downstream fluid processing
- Straightforward scale up to production: conditions identified in the laboratory can be easily scaled up to a tonne per month
- Easy to use: All of Dolomite’s products are designed to quickly connect together without the need for tools. Automation is also available.
- Biomedical – separation of biomolecules for analysis, scaffolds for tissue engineering
- Drug delivery – vehicle for drug delivery (in microparticle form)
- Optics and fluidics – actuators for optics and fluidics
- Biological studies – a model for extracellular matrices
|Polymer material||Method of production||Method of polymerization||Particle Size (CV)||Collateral|
|Agarose||Droplet System (100 µm droplet chip)||Heating/Cooling||35 – 140 µm (0.05)||Agarose Bead Synthesis Application Note|
|Gelatin||Droplet System (190 µm droplet chip)||Cross Linking||150 – 250 µm (0.05)||CHO Cell Encapsulation & Incubation Application Note|
|Polyethyleneglycol -diacrylate (PEGDA)||Droplet System||Photo polymerization||90 – 170 µm (0.05)|
|Alginate||Droplet System (100 µm droplet chip)||Ca+ mediated Precipitation|
Agarose Particle Production
This video is a short summary of the Agarose Bead Synthesis application note, describing the generation of agarose droplets at elevated temperature and subsequent cooling to form solid microparticles.
The system is based around a standardhydrophobic droplet junction chip.